A single-step affinity column for purification of serotype-5 based adeno-associated viral vectors

Mol Ther. 2001 Oct;4(4):372-4. doi: 10.1006/mthe.2001.0462.

Abstract

Here we describe a single-step affinity column for purification of vectors based on adeno-associated virus type 5 (AAV5). A sialic-acid-rich protein called mucin was covalently attached to Sepharose and was found to bind AAV5 vectors. Elution with high salt efficiently recovered highly active vectors of greater purity than what is achieved with CsCl(2) sedimentation.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Cell Line
  • Cesium
  • Chlorides
  • Chromatography, Affinity / methods*
  • Dependovirus / classification*
  • Dependovirus / isolation & purification*
  • Dependovirus / metabolism
  • Dependovirus / physiology
  • Genetic Vectors / isolation & purification*
  • Humans
  • Male
  • Mice
  • Mice, Inbred C57BL
  • Mucins / metabolism
  • Muscle, Skeletal / virology
  • Salts / pharmacology
  • Sepharose / metabolism
  • Ultracentrifugation

Substances

  • Chlorides
  • Mucins
  • Salts
  • Cesium
  • Sepharose
  • cesium chloride