Indication of transposition of a mobile DNA element containing the vat(D) and erm(B) genes in Enterococcus faecium

Antimicrob Agents Chemother. 2001 Nov;45(11):3223-5. doi: 10.1128/AAC.45.11.3223-3225.2001.

Abstract

The vat(D) and erm(B) genes encoding streptogramin resistance in Enterococcus faecium transferred together, and a direct physical link between erm(B) and vat(D) was detected. Both the vat(D) and erm(B) probes hybridized to fragments of different sizes in the donor and transconjugants, which indicated a transposition event.

MeSH terms

  • Anti-Bacterial Agents / pharmacology
  • Blotting, Southern
  • Conjugation, Genetic
  • DNA, Bacterial / chemistry
  • DNA, Bacterial / genetics*
  • Drug Resistance
  • Enterococcus faecium / genetics*
  • Genes, Bacterial / genetics*
  • Methyltransferases / genetics*
  • Molecular Sequence Data
  • Reverse Transcriptase Polymerase Chain Reaction
  • Streptogramins / pharmacology

Substances

  • Anti-Bacterial Agents
  • DNA, Bacterial
  • Streptogramins
  • Methyltransferases
  • rRNA (adenosine-O-2'-)methyltransferase

Associated data

  • GENBANK/AF368302