Abstract
Pasteurella multocida serotype B:2 is the causative agent of haemorrhagic septicaemia, a fatal disease of cattle and buffaloes. Formalin inactivated whole cell bacterin is used to prepare vaccines in India. However, outer membrane proteins (OMPS) of P. multocida were reported to be potential immunogens. The 87-kDa OMP of P. multocida P52, serotype B:2 has been identified as one of the major antigens because this protein reacted with serum of vaccinated animals. The gene omp87, encoding an 87-kDa OMP was amplified and cloned into pBluescript SK(-) vector. This gene was found to localise in a 9.0 kb Hind III fragment of P. multocida genome.
MeSH terms
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Animals
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Antigens, Bacterial / genetics
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Antigens, Bacterial / immunology
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Bacterial Outer Membrane Proteins / genetics*
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Bacterial Outer Membrane Proteins / immunology
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Bacterial Vaccines / genetics
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Bacterial Vaccines / immunology
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Cattle
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Cattle Diseases / immunology
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Cattle Diseases / microbiology*
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Cloning, Molecular
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DNA, Bacterial / genetics
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Hemorrhagic Septicemia / immunology
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Hemorrhagic Septicemia / microbiology
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Hemorrhagic Septicemia / veterinary*
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Pasteurella Infections / immunology
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Pasteurella Infections / microbiology
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Pasteurella Infections / veterinary*
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Pasteurella multocida / genetics*
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Pasteurella multocida / immunology
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Polymerase Chain Reaction
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Restriction Mapping
Substances
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Antigens, Bacterial
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Bacterial Outer Membrane Proteins
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Bacterial Vaccines
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DNA, Bacterial
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Oma87 protein, bacteria