Abstract
Delivering attenuated lentivirus vaccines as proviral DNA would be simple and inexpensive. Inoculation of macaques with wild-type simian immunodeficiency virus strain mac239 (SIV(mac239)) DNA or SIV(mac239) DNA containing a single deletion in the 3' nef-long terminal repeat overlap region (nef/LTR) led to sustained SIV infections and AIDS. Injection of SIV(mac239) DNA containing identical deletions in both the 5' LTR and 3' nef/LTR resulted in attenuated SIV infections and substantial protection against subsequent mucosal SIV(mac251) challenge.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Sequence
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Animals
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Antibodies, Viral / biosynthesis
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Base Sequence
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CD4 Lymphocyte Count
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DNA Primers
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Interferon-gamma / biosynthesis
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Macaca nemestrina
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Molecular Sequence Data
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Mutagenesis
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Plasmids
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RNA, Viral / blood
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Repetitive Sequences, Nucleic Acid
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Simian Immunodeficiency Virus / immunology*
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Vaccines, DNA / administration & dosage*
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Vaccines, DNA / chemistry
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Vaccines, DNA / immunology
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Viral Vaccines / administration & dosage*
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Viral Vaccines / chemistry
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Viral Vaccines / immunology
Substances
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Antibodies, Viral
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DNA Primers
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RNA, Viral
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Vaccines, DNA
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Viral Vaccines
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Interferon-gamma