Suppression of tumor necrosis factor-alpha production and neutrophil infiltration during ischemia-reperfusion injury of the liver after heat shock preconditioning

J Hepatol. 2001 Nov;35(5):619-27. doi: 10.1016/s0168-8278(01)00191-x.

Abstract

Background/aims: Heat shock preconditioning provides the liver with ischemic tolerance. In this study we examined the effects of heat shock preconditioning on hepatic nonparenchymal cells in light of tumor necrosis factor-alpha (TNF-alpha) production and neutrophil infiltration.

Methods: Rats were exposed to heat shock pretreatment at 42 degrees C in the heat shock group (group HS) and at 37 degrees C in the control group (group C). After a 48-h recovery, the left hepatic lobes were given a 90-min ischemia and reperfused. Plasma concentrations of TNF-alpha, cytokine-induced neutrophil chemoattractant (CINC) and alanine aminotransferase (ALT) were measured. Liver tissues were checked for the presence of TNF-alpha mRNA. Histological staining for CINC and polymorphonuclear leukocyte (PMN) was also evaluated.

Results: In group HS, plasma TNF-alpha levels were significantly more suppressed than in group C (P<0.0001). Expressions of TNF-alpha mRNA in the liver was suppressed in group HS. Production of CINC 2 h after reperfusion was reduced in group HS (P<0.05). PMN infiltration was significantly reduced in group HS (P<0.01). In group HS, liver histology revealed less cellular damage and the plasma level of ALT was significantly reduced (P<0.0001).

Conclusions: Heat shock preconditioning suppressed the production of TNF-alpha and CINC in the liver during reperfusion and consequently reduced neutrophil infiltration.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Alanine Transaminase / blood
  • Animals
  • Chemokines, CXC*
  • Chemotactic Factors / blood
  • Enzyme-Linked Immunosorbent Assay
  • Gene Expression Regulation / physiology*
  • Growth Substances / blood
  • Hot Temperature
  • Intercellular Signaling Peptides and Proteins*
  • Ischemic Preconditioning*
  • Liver Circulation / physiology*
  • Male
  • Neutrophils / physiology*
  • Rats
  • Rats, Wistar
  • Reference Values
  • Reperfusion Injury / genetics
  • Reperfusion Injury / physiopathology*
  • Tumor Necrosis Factor-alpha / genetics*

Substances

  • Chemokines, CXC
  • Chemotactic Factors
  • Cxcl3 protein, rat
  • Growth Substances
  • Intercellular Signaling Peptides and Proteins
  • Tumor Necrosis Factor-alpha
  • Alanine Transaminase