Constitutive expression of rice MADS box gene using seed explants in hot pepper (Capsicum annuum L.)

Mol Cells. 2001 Oct 31;12(2):221-6.

Abstract

Two transgenic pepper plants were obtained from 255 seed explants that were infected with Agrobacterium LBA4404 (pGA1209). One of them (PT2) showed morphological change, such as dwarfism and early flowering by the constitutive expression of the rice OsMADS1 gene. The in vitro condition of the plant regeneration has been optimized from hypocotyl explants on a MS medium that was supplemented with zeatin 3 mg/L, IAA 0.3 mg/L for shoot induction. The optimal rooting condition was at NAA 0.3 mg/L. The transformation frequency was 0.8% from the total hypocotyls. DNA and RNA hybridization analyses showed that the introduced gene was integrated and stably expressed in regenerated plants.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Capsicum / genetics*
  • Capsicum / growth & development
  • Gene Expression
  • Genes, Plant*
  • MADS Domain Proteins
  • Oryza / genetics*
  • Phenotype
  • Plant Proteins / genetics*
  • Plants, Genetically Modified
  • Rhizobium / genetics
  • Transcription Factors / genetics*
  • Transformation, Genetic

Substances

  • MADS Domain Proteins
  • Plant Proteins
  • Transcription Factors