Ammocidin, a new apoptosis inducer in Ras-dependent cells from Saccharothrix sp. I. Production, isolation and biological activity

R Murakami; T Tomikawa; K Shin-ya; J Shinozaki; T Kajiura; T Kinoshita; A Miyajima; H Seto; Y Hayakawa

doi:10.7164/antibiotics.54.710

Ammocidin, a new apoptosis inducer in Ras-dependent cells from Saccharothrix sp. I. Production, isolation and biological activity

J Antibiot (Tokyo). 2001 Sep;54(9):710-3. doi: 10.7164/antibiotics.54.710.

Authors

R Murakami¹, T Tomikawa, K Shin-ya, J Shinozaki, T Kajiura, T Kinoshita, A Miyajima, H Seto, Y Hayakawa

Affiliation

¹ Institute of Molecular and Cellular Biosciences, The University of Tokyo, Japan.

PMID: 11714226
DOI: 10.7164/antibiotics.54.710

Abstract

A new apoptosis inducer, ammocidin, was isolated from the culture broth of Saccharothrix sp. AJ9571. Ammocidin induced apoptotic cell death in Ras-dependent Ba/F3-V12 cells with an IC50 of 66 ng/ml. No cell death was observed in IL-3-dependent Ba/F3-V12 cells at less than 100 microg/ml of ammocidin. Ammocidin significantly reduced the phosphorylation level of MAPK and S6K that mediate the anti-apoptotic function of Ras.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Actinomycetales / growth & development
Actinomycetales / metabolism*
Apoptosis / drug effects*
Apoptosis / physiology
Cell Line
Hematopoietic Stem Cells / drug effects*
Humans
Macrolides / chemistry
Macrolides / isolation & purification*
Macrolides / metabolism
Macrolides / pharmacology*
ras Proteins / metabolism

Substances

Macrolides
ammocidin
ras Proteins