Pleckstrin homology domain interacts with Rkp1/Cpc2, a RACK1 homolog, to modulate Pck2-mediated signaling process in Schizosaccharomyces pombe

Biochem Biophys Res Commun. 2001 Dec 21;289(5):987-92. doi: 10.1006/bbrc.2001.6094.

Abstract

Rkp1/Cpc2, a fission yeast RACK1 homolog, interacts with Pck2, a PKC homolog, and is involved in the regulation of pck2-mediated signaling process. The N-terminal region of split pleckstrin homology domain (nPH) in human PLC-gamma1 bound to Rkp1/Cpc2 concomitantly with Pck2. nPH inhibited kinase activity of GST-Pck2 purified from Schizosaccharomyces pombe in vitro. The lethality induced by pck2(+) overexpression was suppressed by coexpression of either rkp1(+) or nPH domain. This result suggests that Rkp1/Cpc2 interacts with PH domain-containing protein and regulates the Pck2-mediated signaling process in S. pombe.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Binding Sites
  • Blood Proteins / chemistry
  • Blood Proteins / metabolism*
  • GTP-Binding Proteins
  • Humans
  • In Vitro Techniques
  • Isoenzymes / chemistry
  • Isoenzymes / metabolism
  • Neoplasm Proteins / metabolism*
  • Phospholipase C gamma
  • Phosphoproteins / chemistry
  • Phosphoproteins / metabolism*
  • Protein Binding
  • Protein Kinase C / metabolism
  • Protein Structure, Tertiary
  • Receptors for Activated C Kinase
  • Receptors, Cell Surface / metabolism*
  • Recombinant Fusion Proteins / chemistry
  • Recombinant Fusion Proteins / metabolism
  • Schizosaccharomyces / metabolism*
  • Schizosaccharomyces pombe Proteins
  • Signal Transduction
  • Type C Phospholipases / chemistry
  • Type C Phospholipases / metabolism

Substances

  • Blood Proteins
  • Isoenzymes
  • Neoplasm Proteins
  • Phosphoproteins
  • RACK1 protein, human
  • Receptors for Activated C Kinase
  • Receptors, Cell Surface
  • Recombinant Fusion Proteins
  • Schizosaccharomyces pombe Proteins
  • cpc2 protein, S pombe
  • platelet protein P47
  • Protein Kinase C
  • Type C Phospholipases
  • Phospholipase C gamma
  • GTP-Binding Proteins