Abstract
The pore-forming microcin E492 was purified by solid-phase extraction and reversed-phase high-pressure liquid chromatography. Its molecular mass was 7,886 Da. The entire 84-amino-acid sequence was determined. There is no postranslational modification in the secreted microcin, and the sequence has homologies with the sequence of the microcin colicin V.
MeSH terms
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Anti-Bacterial Agents / chemistry*
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Anti-Bacterial Agents / isolation & purification
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Bacteriocins / chemistry*
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Bacteriocins / isolation & purification
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Chromatography, High Pressure Liquid
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Colicins / chemistry*
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Molecular Sequence Data
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Molecular Weight
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Protein Conformation
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Sequence Analysis, Protein
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Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
Substances
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Anti-Bacterial Agents
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Bacteriocins
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Colicins
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microcin