With biochemical techniques, the authors preliminarilty examined the dynamics of polyphenols, free amino acids and water-soluble sugars in Gynostemma pentaphyllum collected from different regions and the relationships between them and climate factors. The dynamic patterns of these three types of constituents in stems, blades and shoots under the same environment were different. At different growth phases, stems and blades had different contributions to the constituents. The dynamics of them differed with their environments. Polyphenols, free amino acids and water-soluble sugars might regulate the growth and development of plants. Moreover, they were characterized by plasticity, and the dominant climate factors affecting the dynamics of the constituents varied greatly.