Screening for interacting partners for G alpha i3 and RGS-GAIP using the two-hybrid system

Methods Enzymol. 2002:344:657-73. doi: 10.1016/s0076-6879(02)44747-7.
No abstract available

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Cell Line
  • Cloning, Molecular / methods
  • DNA-Binding Proteins
  • Escherichia coli / genetics
  • Fungal Proteins / genetics
  • Fungal Proteins / metabolism
  • Gene Library
  • Genetic Vectors
  • Glutathione Transferase / genetics
  • Heterotrimeric GTP-Binding Proteins / chemistry
  • Heterotrimeric GTP-Binding Proteins / metabolism*
  • Mammals
  • Phosphoproteins / chemistry
  • Phosphoproteins / metabolism*
  • Plasmids
  • Promoter Regions, Genetic
  • Protein Biosynthesis
  • Protein Multimerization
  • RGS Proteins / chemistry
  • RGS Proteins / metabolism*
  • Recombinant Fusion Proteins / metabolism
  • Saccharomyces cerevisiae / genetics
  • Saccharomyces cerevisiae Proteins*
  • Transcription Factors / genetics
  • Transcription Factors / metabolism
  • Transformation, Genetic
  • Two-Hybrid System Techniques

Substances

  • DNA-Binding Proteins
  • Fungal Proteins
  • GAL4 protein, S cerevisiae
  • Phosphoproteins
  • RGS Proteins
  • Recombinant Fusion Proteins
  • Saccharomyces cerevisiae Proteins
  • Transcription Factors
  • regulator of G-protein signalling 19
  • Glutathione Transferase
  • Heterotrimeric GTP-Binding Proteins