Objective: To define chromosome locus of ATRA-induced gene RA109 and intracellular localization of its encoding protein.
Methods: Chromosome locus was defined by radiation hybrid mapping method and protein localization by green fluorescence protein (GFP) as reporter molecule.
Results: RA109 gene was mapped to chromosome 5q35 and its protein was localized in cell nucleus.
Conclusion: RA109 gene is mapped by radiation hybrid technique to a chromosome region that is rich in disease-related genes. The RA 109-encoding protein is a nucleoprotein.