Continuous antigen delivery from controlled release implants induces significant and anamnestic immune responses

Vaccine. 2002 Jan 15;20(7-8):1089-98. doi: 10.1016/s0264-410x(01)00444-3.

Abstract

Two continuous delivery injectable silicone implants were tested to determine if they were capable of delivering vaccines in a single shot. The Type A implant delivers antigen in vitro over a 1-month-period and the Type B over several months. Vaccination studies in sheep were designed to compare the responses induced by the Type A and B implants, Alzet mini-osmotic pumps and conventional antigen delivery. A model antigen, avidin, was used along with IL-1beta or alum as adjuvants. Sheep were immunised with various formulations and the titre and isotype of the antigen specific antibodies monitored. The Type B implant induced antibody (Ab) titres of greater magnitude and duration than soluble vaccines or the Type A implant with adjuvant, but only if IL-1beta was included in the formulation. Both implants induced antibodies of IgG1 and IgG2 isotype. A memory response to soluble antigen challenge was induced by the Type B+IL-1beta implant, which was predominantly of an IgG1 isotype.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antibody Formation
  • Antigens / administration & dosage*
  • Antigens / immunology
  • Dose-Response Relationship, Immunologic
  • Drug Delivery Systems
  • Drug Implants
  • Female
  • Immunoglobulin Isotypes / blood
  • Infusion Pumps
  • Interleukin-1 / pharmacology
  • Sheep
  • Vaccination
  • Vaccines / administration & dosage*
  • Vaccines / immunology

Substances

  • Antigens
  • Drug Implants
  • Immunoglobulin Isotypes
  • Interleukin-1
  • Vaccines