Alterations in lung collectins in an adaptive allergic immune response

Am J Physiol Lung Cell Mol Physiol. 2002 Mar;282(3):L573-84. doi: 10.1152/ajplung.00117.2001.

Abstract

Although surfactant apoproteins are known to be mediators of innate responses, their relationship to adaptive responses has not been examined extensively. We investigated possible links between surfactant apoproteins and responses to allergens by studying alterations in surfactant apoproteins A, B, and D in a murine model of allergic pulmonary inflammation. Three murine strains (BALB/c, C57BL/6, and 129J) demonstrated increased immunostaining of surfactant apoproteins A and D in nonciliated epithelial cells of noncartilaginous airways after aerosolized challenge. In contrast, surfactant apoprotein B immunostaining was unchanged. Immunoblotting demonstrated increased surfactant A in bronchoalveolar lavage fluid after allergen sensitization and challenge. Surfactant apoprotein A and D induction required T and/or B lymphocyte responses to allergen, since the induction was absent in recombinase-activating gene-deficient mice, which lack functional lymphocytes. We conclude that increased immunoreactivity of two collectins, surfactant apoproteins A and D, occurs within the response to allergen. Our findings support a model in which surfactant apoproteins A and D are important to both innate immunity and adaptive immune responses to allergens.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Adaptation, Physiological / physiology*
  • Animals
  • Antibody Formation / physiology
  • Apoproteins / metabolism
  • B-Lymphocytes / physiology
  • Bronchoalveolar Lavage Fluid / chemistry
  • Carrier Proteins / metabolism*
  • Collectins
  • Genes, RAG-1
  • Glycoproteins / metabolism
  • Hypersensitivity / immunology*
  • Hypersensitivity / metabolism*
  • Immunization
  • Lung / metabolism*
  • Mice
  • Mice, Inbred Strains
  • Mice, Knockout / genetics
  • Ovalbumin / immunology
  • Proteolipids / metabolism
  • Pulmonary Surfactant-Associated Protein D
  • Pulmonary Surfactant-Associated Proteins
  • Pulmonary Surfactants / metabolism
  • T-Lymphocytes / physiology

Substances

  • Apoproteins
  • Carrier Proteins
  • Collectins
  • Glycoproteins
  • Proteolipids
  • Pulmonary Surfactant-Associated Protein D
  • Pulmonary Surfactant-Associated Proteins
  • Pulmonary Surfactants
  • pulmonary surfactant apoprotein
  • Ovalbumin