Objective: In order to investigate the prevalence of Human ehrlichia infectious people in Daxingan Mountains.
Methods: Using PCR detection, followed by sequence analysis to detect EC and Human granulocytic ehrlichia from people in Daxingan Mountains applying the 16S rRNA gene primers of the two agents.
Results: Specific 16S rRNA gene sequences of EC and HGE agent were amplified from human blood specimens of tick-bitted patients and woodsmen. The detected HGE agent 16S rRNA gene (1 433 bp) amplified from a blood specimen of a tick-bitted patient differed in only one base compared with that of an American HGE agent strain.
Conclusion: It was considered that Human ehrlichia infectious people did exist in Daxingan Mountains.