Efficacy of novel culture environments on the ex vivo expansion kinetics of cord blood progenitor cells

Haematologica. 2002 Mar;87(3):320-1.

Abstract

We demonstrate the efficacy of two cytokine-rich supernatants, one from peripheral blood cell cultures stimulated with anti-CD3 and the other from monocyte-conditioned media, in enhancing the rapid (within 6 days) expansion and clonogenicity of CD34+, progenitor cells. We, thereby,increased the generation of mostly myeloid precursors able to support stem cell transplants.

Publication types

  • Letter

MeSH terms

  • Cell Culture Techniques / methods
  • Cell Division
  • Culture Media, Conditioned
  • Fetal Blood / cytology*
  • Humans
  • Kinetics
  • Stem Cells / cytology*

Substances

  • Culture Media, Conditioned