[Modified oligonucleotides containing 1-beta-D-galactopyranosylthymine: synthesis and substrate properties]

B S Ermolinskiĭ; M V Fomicheva; E V Efimtseva; S N Mikhaĭlov; D S Esipov; E F Boldyreva; V G Korobko; A van Aerschot; P Herdewijn

[Modified oligonucleotides containing 1-beta-D-galactopyranosylthymine: synthesis and substrate properties]

Bioorg Khim. 2002 Jan-Feb;28(1):56-63.

[Article in Russian]

Authors

B S Ermolinskiĭ¹, M V Fomicheva, E V Efimtseva, S N Mikhaĭlov, D S Esipov, E F Boldyreva, V G Korobko, A van Aerschot, P Herdewijn

Affiliation

¹ Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, ul. Vavilova 32, Moscow, 117984 Russia.

PMID: 11875975

Abstract

A convenient method of regioselective introduction of 1-beta-D-galactopyranosylthymine into oligonucleotides was developed and the substrate properties of the modified oligonucleotides were investigated in the enzymic reaction of formation and hydrolysis of internucleotide bonds. The English version of the paper.

Publication types

English Abstract

MeSH terms

Biochemistry / methods*
Deoxyribonuclease EcoRI / chemistry
Deoxyribonuclease EcoRI / metabolism
Deoxyribonucleases, Type II Site-Specific / chemistry
Deoxyribonucleases, Type II Site-Specific / metabolism
Galactose / analogs & derivatives
Galactose / chemistry*
Galactose / metabolism*
Hydrolysis
Magnetic Resonance Spectroscopy
Molecular Structure
Oligonucleotides / chemistry*
Oligonucleotides / metabolism*
Structure-Activity Relationship
Thymine / analogs & derivatives
Thymine / chemistry*
Thymine / metabolism*

Substances

1-galactopyranosylthymine
Oligonucleotides
Deoxyribonuclease EcoRI
endodeoxyribonuclease XBAI
Deoxyribonucleases, Type II Site-Specific
GATATC-specific type II deoxyribonucleases
Thymine
Galactose