Low-density lipoprotein reconstituted by pyropheophorbide cholesteryl oleate as target-specific photosensitizer

Bioconjug Chem. 2002 May-Jun;13(3):392-6. doi: 10.1021/bc025516h.

Abstract

To target tumors overexpressing low-density lipoprotein receptors (LDLr), a pyropheophorbide cholesterol oleate conjugate was synthesized and successfully reconstituted into the low-density lipoprotein (LDL) lipid core. Laser scanning confocal microscopy studies demonstrated that this photosensitizer-reconstituted LDL can be internalized via LDLr by human hepatoblastoma G(2) (HepG(2)) tumor cells.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Binding, Competitive
  • Chlorophyll / analogs & derivatives*
  • Chlorophyll / chemistry
  • Chlorophyll / metabolism*
  • Chlorophyll / pharmacology*
  • Cholesterol Esters / chemistry
  • Cholesterol Esters / metabolism*
  • Cholesterol Esters / pharmacology*
  • Endosomes / metabolism
  • Humans
  • Lipoproteins, LDL / metabolism*
  • Lysosomes / metabolism
  • Microscopy, Confocal
  • Microscopy, Fluorescence
  • Photosensitizing Agents / chemistry
  • Photosensitizing Agents / pharmacology*
  • Receptors, LDL / chemistry
  • Receptors, LDL / metabolism*
  • Tumor Cells, Cultured / drug effects*
  • Tumor Cells, Cultured / metabolism

Substances

  • Cholesterol Esters
  • Lipoproteins, LDL
  • Photosensitizing Agents
  • Receptors, LDL
  • Chlorophyll
  • pyropheophorbide a
  • cholesteryl oleate