[Plerosis of cDNA array of normal human nasopharyngeal tissue and nasopharyngeal carcinoma]

Zhonghua Zhong Liu Za Zhi. 2002 Mar;24(2):114-7.
[Article in Chinese]

Abstract

Objective: To compare gene expression gene profile of nasopharyngeal carcinoma (NPC) tissue with that of normal nasopharyngeal tissues by cDNA array and to discuss possible functions of DNA repair-related genes in NPC tissue.

Methods: After hybridization of atlas human cancer cDNA expression array 7742 - 1, atlas hybridization results were analyzed by Atlas Image 1.01 a software package. Using RT-PCR was used to confirm the results.

Results: Of 63 differentially expressed genes in quadrangle C including DNA damage response, repair & recombination-related genes, 6 DNA repair-related genes were up-regulated, 12 were down-regulated.

Conclusion: DNA repair-related genes may be involved in patho-physiological process of nasopharyngeal carcinoma.

Publication types

  • Comparative Study
  • English Abstract

MeSH terms

  • DNA Repair / genetics
  • Gene Expression Profiling*
  • Gene Expression Regulation, Neoplastic
  • Humans
  • Nasopharyngeal Neoplasms / genetics*
  • Nasopharyngeal Neoplasms / pathology
  • Nasopharynx / metabolism*
  • Nasopharynx / pathology
  • Oligonucleotide Array Sequence Analysis / methods*
  • RNA, Neoplasm / genetics
  • Reverse Transcriptase Polymerase Chain Reaction

Substances

  • RNA, Neoplasm