Studies on mass production of transformed Panax ginseng hairy roots in bioreactor

Appl Biochem Biotechnol. 2002 Spring:98-100:1115-27. doi: 10.1385/abab:98-100:1-9:1115.

Abstract

The growth properties of Panax ginseng hairy roots transformed by Agrobacterium rhizogenes were compared between flask and aerated column or stirred bioreactor. In flask cultures, sucrose, initially 30 g/L, was nearly exhausted after 45 d of culture. The pH of the medium dropped from 5.5 to 4.96 after 10 d, but afterward it gradually increased to 6.4. After 45 d, hairy roots grew about 16-folds. The growth rate of hairy roots in air-bubble column or stirred bioreactor cultures was 1.13 (1.11) to 1.23 (1.20) g fresh wt (dry wt)/(g of cells x d), respectively. For both bioreactors, growth was about three times as high as in the flask cultivation.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Air
  • Biomass*
  • Bioreactors*
  • Biotechnology / methods
  • Cells, Cultured
  • Kinetics
  • Panax / growth & development
  • Panax / metabolism*
  • Panax / microbiology
  • Plant Roots / growth & development
  • Plant Roots / metabolism
  • Plant Roots / microbiology
  • Rhizobium / genetics
  • Rhizobium / growth & development
  • Rhizobium / metabolism*
  • Saponins / metabolism*
  • Time Factors

Substances

  • Saponins