TEM-71, a novel plasmid-encoded, extended-spectrum beta-lactamase produced by a clinical isolate of Klebsiella pneumoniae

Antimicrob Agents Chemother. 2002 Jun;46(6):2000-3. doi: 10.1128/AAC.46.6.2000-2003.2002.

Abstract

TEM-71, a novel extended-spectrum beta-lactamase from a Klebsiella pneumoniae clinical isolate, had an isoelectric point of 6.0 and a substrate profile showing preferential hydrolysis of cefotaxime over ceftazidime. It differed from TEM-1 by two substitutions, Gly238Ser and Glu240Lys, and was under the control of the strong P4 promoter.

Publication types

  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Amino Acid Substitution
  • Cefotaxime / metabolism
  • Ceftazidime / metabolism
  • Cephalosporin Resistance / genetics*
  • Cephalosporins / metabolism
  • Cephalosporins / pharmacology
  • DNA, Bacterial / genetics
  • Isoelectric Focusing
  • Kinetics
  • Klebsiella pneumoniae / drug effects
  • Klebsiella pneumoniae / enzymology*
  • Klebsiella pneumoniae / genetics*
  • Microbial Sensitivity Tests
  • Molecular Sequence Data
  • Plasmids / genetics*
  • Reverse Transcriptase Polymerase Chain Reaction
  • Substrate Specificity
  • beta-Lactamases / chemistry
  • beta-Lactamases / genetics*
  • beta-Lactamases / metabolism*

Substances

  • Cephalosporins
  • DNA, Bacterial
  • Ceftazidime
  • TEM-71 beta-lactamase
  • beta-Lactamases
  • Cefotaxime

Associated data

  • GENBANK/AF203816