[Cytokine mRNA expression in vernal keratoconjunctivitis]

Nippon Ganka Gakkai Zasshi. 2002 Jul;106(7):392-7.
[Article in Japanese]

Abstract

Background: Cytokines play an important role in chronic allergic eye disease.

Objectives: We examined how many kinds of cytokines are in giant papillae of vernal keratoconjunctivitis(VKC).

Methods: We resected giant papillae from 6 patients with VKC, and studied mRNA expression of cytokines in them with the reverse transcriptase-polymerase chain reaction(RT-PCR). The cytokines were interleukin(IL)-1 beta, IL-1 receptor antagonist (IL-1 RA), IL-2, IL-4, IL-5, IL-6, IL-8, interferon (INF) gamma, tumor necrosis factor(TNF) alpha, TNF beta, transforming growth factor(TGF) beta 1, and TGF beta 2.

Results: In 5 cases, expression of IL-1 beta was positive. Expression of IL-1 RA, IL-5, IL-6, IL-8, TGF beta 1, and TGF beta 2 was positive in 6 cases. TNF alpha was positive in 4 cases. IL-4 and INF gamma were positive in 3 cases. IL-2 and TNF beta were negative in all patients.

Conclusion: These results suggest that the Th2 cytokines play more important roles than the Th1 cytokines in VKC inflammation.

Publication types

  • English Abstract

MeSH terms

  • Adolescent
  • Adult
  • Child
  • Conjunctivitis, Allergic / metabolism*
  • Cytokines / biosynthesis*
  • Cytokines / genetics
  • Female
  • Humans
  • Male
  • RNA, Messenger / biosynthesis
  • Reverse Transcriptase Polymerase Chain Reaction

Substances

  • Cytokines
  • RNA, Messenger