Validation of a recombinant DNA construct (micro LCR and full-length beta-globin gene) for quantification of human beta-globin expression: application to mutations in the promoter, intronic, and 5'- and 3'-untranslated regions of the human beta-globin gene

MeSH terms

• 3' Untranslated Regions
• 5' Untranslated Regions
• Animals
• DNA, Recombinant / analysis*
• Globins / genetics
• Globins / metabolism*
• Humans
• Introns
• Mice
• Mutation
• Promoter Regions, Genetic
• Reverse Transcriptase Polymerase Chain Reaction
• Tumor Cells, Cultured
• beta-Thalassemia / metabolism