lal-1: a differentially expressed novel gene during proliferation in liver regeneration and in hepatoma cells

Genes Cells. 2002 Nov;7(11):1183-90. doi: 10.1046/j.1365-2443.2002.00593.x.

Abstract

Background: During liver regeneration, 95% of the resting hepatocytes enter in G1/S phase of the cell cycle. A number of hormones, growth factors and cytokines were identified that activate signal transduction pathways playing a primary role in hepatocyte proliferation. A wide and representative cDNA library containing 1.5 x 106 independent clones was constructed from regenerating liver in order to identify and characterize gene the products which play a crucial role in the first hours of the proliferative process of liver regeneration.

Results: A novel gene expressed in liver regeneration was cloned by subtractive hybridization. The putative protein displays in the N'-terminal a annexin-like domain and an aminopeptidase domain. We named the novel gene Liver Annexin Like-1 (lal-1). The lal-1 gene is modulated during liver regeneration, in hepatoma cells following physiological stimulation and after cAMP induction.

Conclusion: The results indicate that lal-1 is involved in liver regeneration and that its expression is finely regulated during proliferative process. The isolation of lal-1 paves the way for a further characterization helping to assess lal-1 involvement in cell function and proliferation.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Annexins / genetics*
  • Annexins / physiology
  • Carcinoma, Hepatocellular / genetics*
  • DNA, Neoplasm / genetics
  • Fluorescent Antibody Technique
  • Gene Expression Regulation, Neoplastic*
  • Liver Neoplasms, Experimental / genetics*
  • Liver Regeneration / genetics*
  • Liver Regeneration / physiology
  • Microscopy, Fluorescence
  • Organ Specificity
  • Rats
  • Sequence Analysis, DNA

Substances

  • Annexins
  • Cpq protein, rat
  • DNA, Neoplasm