Use of polymerase chain reaction for diagnosis of disseminated adenovirus infection

Pediatr Infect Dis J. 2002 Dec;21(12):1176-8. doi: 10.1097/00006454-200212000-00023.

Abstract

We report a fatal case of disseminated adenovirus infection with fulminant hepatic failure in an 8-month-old child after peripheral blood stem cell transplantation. The virus was identified in blood, urine, respiratory aspirate and stool samples and was typed as adenovirus type 2 through PCR and sequencing of part of the hexon gene, with the use of degenerated consensus primers.

Publication types

  • Case Reports

MeSH terms

  • Adenoviridae Infections / diagnosis*
  • Adenoviridae Infections / therapy
  • Adenoviruses, Human / isolation & purification*
  • DNA, Viral / blood*
  • Disease Progression
  • Fatal Outcome
  • Hematopoietic Stem Cell Transplantation / adverse effects
  • Humans
  • Infant
  • Leukemia, Myelomonocytic, Acute / diagnosis
  • Leukemia, Myelomonocytic, Acute / immunology
  • Leukemia, Myelomonocytic, Acute / therapy
  • Male
  • Multiple Organ Failure
  • Polymerase Chain Reaction / methods*
  • Sensitivity and Specificity
  • Severity of Illness Index
  • Viremia / diagnosis*
  • Viremia / therapy

Substances

  • DNA, Viral