Abstract
From necrotic tissue of a Nashi pear tree, 24 Erwinia pyrifoliae strains, found to be identical by pulsed-field gel electrophoresis analysis, were isolated. Thirteen strains were not virulent on immature pears and did not induce a hypersensitive response in tobacco leaves. The defective gene hrpL was complemented with intact genes from E. pyrifoliae and Erwinia amylovora.
MeSH terms
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Amino Acid Sequence
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Bacterial Proteins / chemistry
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Bacterial Proteins / genetics*
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Base Sequence
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DNA-Binding Proteins*
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Electrophoresis, Gel, Pulsed-Field
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Erwinia / genetics
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Erwinia / growth & development
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Erwinia / pathogenicity*
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Genetic Complementation Test
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Molecular Sequence Data
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Nicotiana / microbiology
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Plant Diseases / microbiology*
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Plant Leaves / microbiology
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Pyrus / microbiology*
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Sequence Analysis, DNA
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Sigma Factor*
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Virulence
Substances
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Bacterial Proteins
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DNA-Binding Proteins
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HrpL protein, Pseudomonas syringae
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Sigma Factor