Objective: The objective of this study is to construct prokaryotic expressing plasmids carrying A region of S. mutans pac gene.
Methods: The A region of pac gene was amplified by using PCR from plasmid pPC41 containing S. mutans surface protein pac gene. The new expression plasmid was constructed by using A region of pac gene and expressing plasmids pET17-b and directed cloning technique. The recombinant plasmid was identified by agarose gel electrophoresis after it was cut by using BamHI and EcoRV.
Results: The expressing plasmid carrying pac gene A region was acquired.
Conclusion: The recombinant plasmid provides useful experimental materials for further research work.