Independent subsets of amplified fragments from the genome of Neisseria meningitidis identify the same invasive clones of ET37 and ET5

J Med Microbiol. 2003 Feb;52(Pt 2):151-154. doi: 10.1099/jmm.0.05012-0.

Abstract

To determine whether fluorescent amplified-fragment length polymorphism (FAFLP) analysis is an unbiased genome sampling technique, data were analysed from three different primer combinations, amplifying three independent fragment subsets from 123 isolates of Neisseria meningitidis. Using these data, dendrograms were generated with near-identical topologies that identified the same invasive clones of ET37 and ET5 and also identified the same outbreak clusters.

MeSH terms

  • Bacterial Typing Techniques / methods
  • Base Sequence
  • DNA, Bacterial / genetics
  • DNA, Bacterial / isolation & purification
  • Disease Outbreaks*
  • Fluorescent Dyes
  • Gene Amplification*
  • Genome, Bacterial
  • Humans
  • Meningococcal Infections / epidemiology
  • Meningococcal Infections / microbiology*
  • Neisseria meningitidis / classification*
  • Neisseria meningitidis / genetics
  • Phylogeny
  • Polymerase Chain Reaction
  • Polymorphism, Restriction Fragment Length*
  • United Kingdom / epidemiology

Substances

  • DNA, Bacterial
  • Fluorescent Dyes