Structure of an acidic polysaccharide from the marine bacterium Pseudoalteromonas flavipulchra NCIMB 2033(T)

Jimmy Muldoon; Andrei V Perepelov; Alexander S Shashkov; Evgeny L Nazarenko; Vladimir A Zubkov; Raisa P Gorshkova; Elena P Ivanova; Natalya M Gorshkova; Yuriy A Knirel; Angela V Savage

doi:10.1016/s0008-6215(02)00483-4

Structure of an acidic polysaccharide from the marine bacterium Pseudoalteromonas flavipulchra NCIMB 2033(T)

Carbohydr Res. 2003 Feb 14;338(5):459-62. doi: 10.1016/s0008-6215(02)00483-4.

Authors

Jimmy Muldoon¹, Andrei V Perepelov, Alexander S Shashkov, Evgeny L Nazarenko, Vladimir A Zubkov, Raisa P Gorshkova, Elena P Ivanova, Natalya M Gorshkova, Yuriy A Knirel, Angela V Savage

Affiliation

¹ Department of Chemistry, National University of Ireland, University Road, Galway, Ireland.

PMID: 12559750
DOI: 10.1016/s0008-6215(02)00483-4

Abstract

An acidic polysaccharide was isolated from Pseudoalteromonas flavipulchra type strain NCIMB 2033(T) and found to consist of 6-deoxy-L-talose (L-6dTal), D-galactose and 3-deoxy-D-manno-oct-2-ulosonic acid (Kdo). The identities of the monosaccharides were ascertained by sugar analysis and 1D 1H and 13C NMR spectroscopy in conjunction with 2D COSY, TOCSY, ROESY and 1H, 13C HMQC experiments, which enabled determination of the following structure of the trisaccharide repeating unit of the polysaccharide:-->3)-alpha-L-6dTalp4Ac-(1-->3)-beta-D-Galp-(1-->7)-alpha-Kdop-(2-->.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Deoxy Sugars / chemistry
Galactose / chemistry
Hexoses / chemistry
Magnetic Resonance Spectroscopy
Polysaccharides / chemistry*
Pseudoalteromonas / chemistry*
Sugar Acids / chemistry

Substances

Deoxy Sugars
Hexoses
Polysaccharides
Sugar Acids
6-deoxytalose
Galactose
3-deoxy-manno-oct-2-ulopyranosonic acid