Identification and quantification of ovine gammaherpesvirus 2 DNA in fresh and stored tissues of pigs with symptoms of porcine malignant catarrhal fever

J Clin Microbiol. 2003 Feb;41(2):900-4. doi: 10.1128/JCM.41.2.900-904.2003.

Abstract

Cases of porcine malignant catarrhal fever were analyzed by a combination of identification and quantitation of ovine gammaherpesvirus 2 DNA in a variety of paraffin-embedded tissues from diseased pigs, serology, and exclusion of primary porcine gammaherpesviruses. In spite of reduced signal due to fixation and paraffin embedding, ovine gammaherpesvirus 2 DNA in pig brains exceeded the amounts found in sheep brains by orders of magnitude.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Brain / pathology
  • Brain / virology
  • DNA, Viral / analysis
  • Gammaherpesvirinae / isolation & purification*
  • Gammaherpesvirinae / physiology
  • Herpesviridae Infections / veterinary*
  • Histological Techniques
  • Malignant Catarrh / pathology
  • Malignant Catarrh / virology*
  • Sheep
  • Sheep Diseases / virology*
  • Swine
  • Swine Diseases / virology*
  • Viral Load

Substances

  • DNA, Viral