Abstract
The efficiency of CD8 memory response relies partially on the modification of cellular functional capacities. To identify effector functions that can be modified following priming, we have compared the chemokines produced by naive and memory CD8 T cells. Our results show that in contrast to naive cells, resting memory CD8 T cells contain high levels of RANTES mRNA. As a result, they have the capacity to rapidly secrete RANTES upon ex vivo antigenic stimulation. In contrast to that of IFN-gamma, RANTES secretion is mainly due to the translation of the pre-existing mRNA.
Publication types
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Comparative Study
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Antigens / pharmacology
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CD8-Positive T-Lymphocytes / immunology
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CD8-Positive T-Lymphocytes / metabolism*
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CD8-Positive T-Lymphocytes / virology
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Cells, Cultured
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Chemokine CCL5 / biosynthesis
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Chemokine CCL5 / genetics
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Chemokine CCL5 / metabolism*
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Immunologic Memory* / genetics
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Interferon-gamma / biosynthesis
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Interferon-gamma / genetics
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Interphase / genetics
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Interphase / immunology*
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Kinetics
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Lymphocyte Activation* / genetics
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Mice
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Mice, Inbred C57BL
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Mice, Transgenic
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Peptides / immunology
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Peptides / pharmacology
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RNA, Messenger / biosynthesis
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T-Lymphocyte Subsets / immunology
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T-Lymphocyte Subsets / metabolism*
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T-Lymphocyte Subsets / virology
Substances
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Antigens
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Chemokine CCL5
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Peptides
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RNA, Messenger
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Interferon-gamma