Aim: To study the chemical constituents of Pleurospermum rivulorum.
Methods: To separate the constituents of P. rivulorum by using various kinds of chromatography and identify their structures on the basis of spectral analysis.
Results: Four glucosides were isolated from P. rivulorum. Their structures were established as marmesinin (I), tert-O-beta-D-glucopyranosyl-heraclenol (II), 1'-O-beta-D-glucopyranosyl (2R, 3S)-3-hydroxynodakenetin (III) and 8-hydroxy-5-O-beta-D-glucopyranosyl-psoralen (IV) based on the analysis of spectral data of IR, UV, MS, 1D and 2D-NMR.
Conclusion: Compounds III and IV are new compounds. All of the four compounds were isolated from this plant for the first time.