Reduced tumorigenicity of murine leukemia cells expressing protein-tyrosine phosphatase, PTPepsilon C

Oncogene. 2003 Mar 27;22(12):1758-62. doi: 10.1038/sj.onc.1206267.

Abstract

Recently, we reported that a cytosolic isoform of protein-tyrosine phosphatase epsilon (PTP epsilon C), when overexpressed, inhibits terminal differentiation and apoptosis of murine M1 myeloblastic leukemia cells induced by interleukin-6. To determine whether these observed effects in vitro correspond to a tumorigenicity of PTP epsilon C-expresser (M1- epsilon C) cells in vivo, parent M1 and M1- epsilon C cells were intravenously inoculated into scid or nude mice, and survival of mice receiving these cell lines was monitored. Unexpectedly, both scid and nude mice inoculated with M1- epsilon C cells showed significantly prolonged survival time than those receiving parent M1 cells. While parent M1 cells inoculated by intravenous injection formed metastatic tumors in the spleen, expression of PTP epsilon C suppressed tumor development in the spleen. The results suggest a suppressive role of PTP epsilon C in tumorigenesis.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Leukemia, Experimental / enzymology*
  • Leukemia, Experimental / pathology
  • Leukemia, Myeloid, Acute / enzymology*
  • Leukemia, Myeloid, Acute / pathology
  • Mice
  • Mice, Nude
  • Mice, SCID
  • Protein Tyrosine Phosphatases / metabolism*
  • Tumor Cells, Cultured

Substances

  • Protein Tyrosine Phosphatases