Isolation and characterisation of the phospholipase B gene of Cryptococcus neoformans var. gattii

FEMS Yeast Res. 2002 Dec;2(4):551-61. doi: 10.1111/j.1567-1364.2002.tb00122.x.

Abstract

Cryptococcus neoformans var. gattii (serotypes B and C) is a human pathogen, ecologically, biochemically, clinically and genetically different from C. neoformans var. grubii (serotype A) and C. neoformans var. neoformans (serotype D). The phospholipase B (PLB1) gene from serotypes B and C was isolated and characterised. It resembled the serotype A and D genes, with an overall sequence homology of more than 85%. The respective open reading frames were 2236 bp (serotype B) and 2239 bp (serotype C) in length. Each contained six introns and encoded a 68-kDa protein destined for secretion. PLB1 was located on the second smallest chromosome in both serotypes. Gene expression, measured as mRNA, was not regulated by temperature, pH or exogenous nutrients.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Base Sequence
  • Cryptococcus neoformans / enzymology
  • Cryptococcus neoformans / genetics*
  • DNA, Fungal / analysis
  • Genes, Bacterial*
  • Hydrophobic and Hydrophilic Interactions
  • Lysophospholipase / genetics*
  • Lysophospholipase / isolation & purification
  • Lysophospholipase / metabolism
  • Molecular Sequence Data
  • Phylogeny
  • Sequence Alignment
  • Serotyping

Substances

  • DNA, Fungal
  • Lysophospholipase