Abstract
Four hundred and twenty-two calves were examined for intestinal carriage of Shiga toxin-producing Escherichia coli O157:H7 using conventional plating. Two (0.5%) E. coli O157 were recovered. They were compared with 96 Argentine strains of different origin by pulsed-field gel electrophoresis, phage typing and PCR-RFLP of stx2 genes. One strain isolated from a calf, was closely related with 18 strains of clinical origin.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Argentina / epidemiology
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Bacteriophage Typing / veterinary
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Cattle
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Cattle Diseases / epidemiology
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Cattle Diseases / microbiology*
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Cluster Analysis
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DNA, Bacterial / chemistry
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DNA, Bacterial / genetics
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Electrophoresis, Gel, Pulsed-Field / veterinary
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Escherichia coli Infections / epidemiology
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Escherichia coli Infections / microbiology
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Escherichia coli Infections / veterinary*
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Escherichia coli O157 / genetics
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Escherichia coli O157 / isolation & purification*
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Escherichia coli O157 / metabolism
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Feces / microbiology
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Polymerase Chain Reaction / veterinary
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Polymorphism, Restriction Fragment Length
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Prevalence
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Shiga Toxins / biosynthesis*
Substances
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DNA, Bacterial
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Shiga Toxins