The yggH gene of Escherichia coli encodes a tRNA (m7G46) methyltransferase

J Bacteriol. 2003 May;185(10):3238-43. doi: 10.1128/JB.185.10.3238-3243.2003.

Abstract

We cloned, expressed, and purified the Escherichia coli YggH protein and show that it catalyzes the S-adenosyl-L-methionine-dependent formation of N(7)-methylguanosine at position 46 (m(7)G46) in tRNA. Additionally, we generated an E. coli strain with a disrupted yggH gene and show that the mutant strain lacks tRNA (m(7)G46) methyltransferase activity.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Base Sequence
  • Cell Division / genetics
  • Cloning, Molecular
  • Escherichia coli / genetics
  • Escherichia coli / growth & development
  • Escherichia coli Proteins / genetics*
  • Escherichia coli Proteins / metabolism*
  • Guanosine / analogs & derivatives*
  • Guanosine / metabolism
  • Molecular Sequence Data
  • Multigene Family
  • RNA, Transfer / chemistry
  • RNA, Transfer / metabolism
  • Recombinant Proteins / genetics
  • Recombinant Proteins / isolation & purification
  • Recombinant Proteins / metabolism
  • S-Adenosylmethionine / metabolism
  • Sequence Analysis
  • Sequence Homology, Amino Acid
  • tRNA Methyltransferases / genetics*
  • tRNA Methyltransferases / metabolism*

Substances

  • Escherichia coli Proteins
  • Recombinant Proteins
  • Guanosine
  • 7-methylguanosine
  • S-Adenosylmethionine
  • RNA, Transfer
  • YggH protein, E coli
  • tRNA Methyltransferases