Abstract
We have identified IC53-2, a human homologue of the rat C53 gene from a human placenta cDNA library (GeneBank Accession No.AF217982). IC53-2 can bind to the CDK5 activator p35 by in vitro association assay. IC53-2 is mapped to human chromosome 17q21.31. The IC53-2 transcript is highly expressed in kidney, liver, skeletal muscle and placenta. It is abundantly expressed in SMMC-7721, C-33A, 3AO, A431 and MCF-7 cancer cell lines by RT-PCR assay. Stable transfection of IC53-2 cDNA into the hepatocellular carcinoma SMMC-7721 cell remarkably stimulates its growth in vitro. The above results indicate that IC53-2 is a novel human gene, which may be involved in the regulation of cell proliferation.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Carcinoma, Hepatocellular / genetics*
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Carcinoma, Hepatocellular / metabolism
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Carrier Proteins / genetics
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Carrier Proteins / isolation & purification*
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Cell Cycle Proteins
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Cell Division / genetics*
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Cell Transformation, Neoplastic / genetics*
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Cell Transformation, Neoplastic / metabolism
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Chromosomes, Human, Pair 17
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Cloning, Molecular
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Cyclin-Dependent Kinase 5
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Cyclin-Dependent Kinases / genetics*
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Gene Expression Regulation, Neoplastic / genetics
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HeLa Cells
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Humans
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Intracellular Signaling Peptides and Proteins*
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Molecular Sequence Data
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Nerve Tissue Proteins / genetics*
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Nerve Tissue Proteins / isolation & purification*
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Protein Binding / genetics
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Protein Isoforms / genetics
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Sequence Homology, Amino Acid
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Sequence Homology, Nucleic Acid
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Transfection
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Tumor Suppressor Proteins
Substances
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CDK5RAP3 protein, human
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Carrier Proteins
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Cell Cycle Proteins
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Intracellular Signaling Peptides and Proteins
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Nerve Tissue Proteins
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Protein Isoforms
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Tumor Suppressor Proteins
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neuronal Cdk5 activator (p25-p35)
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Cyclin-Dependent Kinase 5
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CDK5 protein, human
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Cyclin-Dependent Kinases