Scanning electron microscopy (SEM) and atomic force microscopy (AFM) have been applied to the imagery of solid lipid nanoparticles (SLNs) formulated from an amphiphilic cyclodextrin, 2,3-di-o-alkanoyl-beta-cyclodextrin, beta-CD21C6. Comparison of the results shows that the vacuum drying technique used in sample preparation for SEM causes shrinkage in the size of the SLNs, whereas the deposition method used for AFM causes the SLNs to form small clusters. The hydrodynamic diameter determined from photon correlation spectroscopy (PCS) is 359+/-15 nm and the zeta potential is -25 mV.