Cloning and characterization of a blood coagulation factor IX-binding protein from the venom of Trimeresurus stejnegeri

Toxicon. 2003 Jun;41(7):765-72. doi: 10.1016/s0041-0101(03)00029-1.

Abstract

A blood coagulation factor IX-binding protein (TSV-FIX-BP) was isolated from the snake venom of Trimeresurus stejnegeri. On SDS-polyacrylamide gel electrophoresis, TSV-FIX-BP showed a single band with an apparent molecular weight of 23,000 under non-reducing conditions, and two distinct bands with apparent molecular weights of 14,800 and 14,000 under reducing conditions. cDNA clones containing the coding sequences of TSV-FIX-BP were isolated and sequenced to determine the structure of the precursors of TSV-FIX-BP subunits. The deduced amino acid sequences of two subunits of TSV-FIX-BP were confirmed by N-terminal protein sequencing and trypsin-digested peptide mass fingerprinting. TSV-FIX-BP was a non-enzymatic C-type lectin-like anti-coagulant. The anti-coagulant activity of TSV-FIX-BP was mainly caused by its dose dependent interaction with blood coagulation factor IX but not with blood coagulation factor X.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Carrier Proteins / chemistry
  • Carrier Proteins / genetics*
  • Carrier Proteins / toxicity*
  • Cloning, Molecular
  • Crotalid Venoms / chemistry
  • Crotalid Venoms / genetics*
  • Crotalid Venoms / toxicity*
  • DNA, Complementary / genetics*
  • Dose-Response Relationship, Drug
  • Electrophoresis, Polyacrylamide Gel
  • Factor IX / drug effects*
  • Gene Library
  • Molecular Sequence Data
  • Reptilian Proteins*
  • Sequence Alignment
  • Sequence Homology
  • Trimeresurus

Substances

  • Carrier Proteins
  • Crotalid Venoms
  • DNA, Complementary
  • Reptilian Proteins
  • Trimeresurus venoms
  • factor IX-binding protein, Trimeresurus flavoviridis
  • Factor IX