Abstract
A quantitative spectrophotometric method was developed to measure the removal and killing efficacy of antibiofilm agents. Biofilms of Pseudomonas aeruginosa or Staphylococcus epidermidis were grown in 96-well plates, treated with an agent, then stained with either the biomass indicator crystal violet or the respiratory indicator 5-cyano-2,3-ditolyl tetrazolium chloride. This rapid screening method is sensitive enough to elucidate concentration-response relationships as well as differences between species responses to treatments. Using these assays, agents can be ranked by their ability to remove or kill biofilm.
Publication types
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Evaluation Study
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Research Support, U.S. Gov't, Non-P.H.S.
MeSH terms
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Biofilms / drug effects*
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Biofilms / growth & development
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Colony Count, Microbial
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Disinfectants / administration & dosage
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Disinfection*
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Dose-Response Relationship, Drug
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Microbial Sensitivity Tests / methods*
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Pseudomonas aeruginosa / drug effects*
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Pseudomonas aeruginosa / growth & development
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Sodium Hypochlorite / pharmacology
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Spectrophotometry
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Staining and Labeling / methods
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Staphylococcus epidermidis / drug effects*
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Staphylococcus epidermidis / growth & development
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Tetrazolium Salts / chemistry
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Urea / pharmacology
Substances
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Disinfectants
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Tetrazolium Salts
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5-cyano-2,3-ditolyltetrazolium
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Urea
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Sodium Hypochlorite