Evaluation of the epidemiologic utility of secondary typing methods for differentiation of Mycobacterium tuberculosis isolates

J Clin Microbiol. 2003 Jun;41(6):2683-5. doi: 10.1128/JCM.41.6.2683-2685.2003.

Abstract

Spoligotyping and mycobacterial interspersed repetitive unit-variable-number tandem repeat analysis (MIRU-VNTR) were evaluated for the ability to differentiate 64 Mycobacterium tuberculosis isolates from 10 IS6110-defined clusters. MIRU-VNTR performed slightly better than spoligotyping in reducing the number of clustered isolates and the sizes of the clusters. All epidemiologically related isolates remained clustered by MIRU-VNTR but not by spoligotyping.

Publication types

  • Comparative Study
  • Evaluation Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Bacterial Typing Techniques*
  • DNA Transposable Elements
  • DNA, Bacterial
  • Genotype
  • Humans
  • Minisatellite Repeats / genetics
  • Mycobacterium tuberculosis / classification*
  • Mycobacterium tuberculosis / genetics*
  • Mycobacterium tuberculosis / isolation & purification
  • Oligodeoxyribonucleotides / analysis
  • Polymorphism, Restriction Fragment Length
  • Repetitive Sequences, Nucleic Acid / genetics
  • Tuberculosis / epidemiology*
  • Tuberculosis / microbiology

Substances

  • DNA Transposable Elements
  • DNA, Bacterial
  • Oligodeoxyribonucleotides