Human polynucleotide phosphorylase, hPNPase, is localized in mitochondria

Jan Piwowarski; Pawel Grzechnik; Andrzej Dziembowski; Aleksandra Dmochowska; Michal Minczuk; Piotr P Stepien

doi:10.1016/s0022-2836(03)00528-x

Human polynucleotide phosphorylase, hPNPase, is localized in mitochondria

J Mol Biol. 2003 Jun 20;329(5):853-7. doi: 10.1016/s0022-2836(03)00528-x.

Authors

Jan Piwowarski¹, Pawel Grzechnik, Andrzej Dziembowski, Aleksandra Dmochowska, Michal Minczuk, Piotr P Stepien

Affiliation

¹ Department of Genetics, Warsaw University and Institute of Biochemistry and Biophysics, Polish Academy of Sciences, ul. Pawinskiego 5A, 02-106 Warsaw, Poland.

PMID: 12798676
DOI: 10.1016/s0022-2836(03)00528-x

Abstract

The human gene encoding a polynucleotide phosphorylase (hPNPase) has been recently identified as strongly up-regulated in two processes leading to irreversible arrest of cell division: progeroid senescence and terminal differentiation. Here, we demonstrate that the hPNPase is localized in mitochondria. Our finding suggests the involvement of mitochondrial RNA metabolism in cellular senescence.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Cloning, Molecular
Cytoplasm / metabolism
Escherichia coli / genetics
HeLa Cells
Humans
Microscopy, Fluorescence
Mitochondria / enzymology
Mitochondria / metabolism*
Peptide Fragments / metabolism
Polyribonucleotide Nucleotidyltransferase / genetics
Polyribonucleotide Nucleotidyltransferase / metabolism*
Protein Transport
Recombinant Proteins / genetics
Recombinant Proteins / metabolism

Substances

Peptide Fragments
Recombinant Proteins
Polyribonucleotide Nucleotidyltransferase