Comparative testing of liposomal and aqueous formulations of povidone-iodine for their angioirritative potential at the chorioallantoic membrane of ex ovo cultivated chick embryos

Dermatology. 2003;207(1):43-7. doi: 10.1159/000070940.

Abstract

Background: Due to its excellent microbicidal activity, povidone-iodine (PVP-I) is used for local anti-infective treatment, especially for wound antisepsis of the skin. Previous studies have shown that liposomal PVP-I formulations are less cytotoxic and that a special liposomal PVP-I hydrogel provides wound healing efficacy.

Objective: In the present study, the angiotoxic activity of the liposomal and the aqueous PVP-I formulations were comparatively tested at the chorioallantoic membrane (CAM) of chick embryos.

Methods: Test preparations were administered onto the CAM of ex ovo cultivated chick embryos. At different time intervals, the local irritation of the blood vessels was examined microscopically and assigned to a score of vascular injury.

Results: Angioirritative reactions of the CAM were manifest as coagulation, vascular congestion and hemorrhages. The liposomal formulations containing 0.1, 0.5 or 1% PVP-I were found to be significantly less angiotoxic than the corresponding aqueous formulations.

Conclusion: The angioirritative potential of PVP-I is significantly reduced by its liposomal encapsulation.

Publication types

  • Comparative Study

MeSH terms

  • Animals
  • Anti-Infective Agents, Local / adverse effects*
  • Anti-Infective Agents, Local / pharmacology*
  • Chemistry, Pharmaceutical
  • Chick Embryo
  • Dose-Response Relationship, Drug
  • Drug Delivery Systems
  • Extraembryonic Membranes / drug effects*
  • Hemorrhage / chemically induced*
  • Hemorrhage / physiopathology
  • Liposomes
  • Models, Animal
  • Povidone-Iodine / adverse effects*
  • Povidone-Iodine / pharmacology*
  • Probability
  • Sensitivity and Specificity
  • Water

Substances

  • Anti-Infective Agents, Local
  • Liposomes
  • Water
  • Povidone-Iodine