Abstract
B7 costimulation plays a major role in herpes simplex virus (HSV) immunity. Effects of immunoregulatory cytokines (interferon-gamma [IFN-gamma] and interleukin-10 [IL-10]) and HSV antigens on B7-1 (CD80) and B7-2 (CD86) expression on monocytes during HSV-2 infection were investigated using flow cytometry. Our results demonstrate that HSV-2 infection effects the expression of B7 isoforms (B7-1 and B7-2) on monocytes in two ways, with opposing outcomes. It abrogates the IFN-gamma upregulation of B7-1 and B7-2 and also augments expression of B7-1 and B7-2 on monocytes through an IFN-gamma independent mechanism. The clinical significance of these opposing effects may be related to the pathogenesis of recurrent HSV disease in immunocompetent hosts.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Antigens, CD / immunology*
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Antigens, CD / metabolism*
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Antigens, Viral / immunology*
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B7-1 Antigen / immunology
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B7-1 Antigen / metabolism*
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B7-2 Antigen
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Cells, Cultured
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Flow Cytometry
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Herpes Genitalis / immunology*
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Herpes Genitalis / virology
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Herpesvirus 1, Human / immunology
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Herpesvirus 2, Human / immunology*
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Humans
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Interferon-gamma / immunology*
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Interleukin-10 / immunology
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Membrane Glycoproteins / immunology*
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Membrane Glycoproteins / metabolism*
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Models, Immunological
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Monocytes / immunology*
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Monocytes / virology
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T-Lymphocytes / immunology
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Up-Regulation
Substances
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Antigens, CD
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Antigens, Viral
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B7-1 Antigen
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B7-2 Antigen
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CD86 protein, human
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Membrane Glycoproteins
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Interleukin-10
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Interferon-gamma