Quantification of doxorubicin and validation of reversal effect of tea polyphenols on multidrug resistance in human carcinoma cells

Biotechnol Lett. 2003 Feb;25(4):291-4. doi: 10.1023/a:1022343832525.

Abstract

HPLC was used to analyze doxorubicin in multidrug-resistance (MDR) human carcinoma cells. This method is novel, simple, sensitive, linear, accurate and precise. The minimal detectable concentration is 0.2 microg ml(-1). The reversal effects of tea polyphenols on MDR are elucidated by this method. The results indicate that the tea polyphenol, (-)-epigallocatechin gallate, is a potential modulator of MDR.

Publication types

  • Evaluation Study
  • Research Support, Non-U.S. Gov't
  • Validation Study

MeSH terms

  • Carcinoma, Squamous Cell / metabolism*
  • Carcinoma, Squamous Cell / pathology
  • Carcinoma, Squamous Cell / physiopathology
  • Catechin / analogs & derivatives*
  • Catechin / pharmacology*
  • Cell Division / drug effects
  • Cell Survival / drug effects
  • Chromatography, High Pressure Liquid / methods
  • Doxorubicin / analysis*
  • Doxorubicin / pharmacology*
  • Drug Resistance, Multiple*
  • Flavonoids*
  • Humans
  • Mouth Neoplasms / metabolism
  • Mouth Neoplasms / physiopathology
  • Phenols / pharmacology*
  • Polymers / pharmacology*
  • Tea / chemistry
  • Tumor Cells, Cultured

Substances

  • Flavonoids
  • Phenols
  • Polymers
  • Tea
  • Doxorubicin
  • Catechin
  • epigallocatechin gallate