Objective: To investigate whether As2O3 induces tumor cell apoptosis and cell cycle arrest.
Methods: DNA agarose gel electrophoresis, flowcytometry analysis, RT-PCR, and Western blot.
Results: As2O3 induced G2 + M arrest of tumor cells such as GLC-82, MGC-803, SGC-7901, Eca109 and HeLa cells before inducing apoptosis and decreased c-myc gene expression, whereas caused G1 block of HPV16 DNA immortalized cervical epithelial HCE16/3 cells and did not affect c-myc expression.
Conclusions: As2O3 caused apoptosis was related with cell cycle arrest. However, the phase of arrest of tumor cells was different from the pre-tumor cell HCE16/3 cells, which could be related to the change of c-myc gene expression.