[Deletion mutation of beta-amyloid precursor protein decreases the secretion of beta-amyloid in SH-SY5Y neuroblastoma cells]

Qinqin Zha; Yan Ruan; Zhonghua Liu; Lianyuan Cao; Dai Zhang

[Deletion mutation of beta-amyloid precursor protein decreases the secretion of beta-amyloid in SH-SY5Y neuroblastoma cells]

Beijing Da Xue Xue Bao Yi Xue Ban. 2003 Jun 18;35(3):261-5.

[Article in Chinese]

Authors

Qinqin Zha¹, Yan Ruan, Zhonghua Liu, Lianyuan Cao, Dai Zhang

Affiliation

¹ Institute of Mental Health, Peking University, Beijing 100083, China.

PMID: 12914241

Abstract

Objective: To explore the effect of A beta secretion lacking exons 2 and 3 of APP695 (delta APP695) on SH-SY5Y neuroblastoma cells.

Methods: APP695 cDNA and delta APP695 cDNA eukaryotic expression vectors were constructed by gene recombination. Immunoprecipitation Western blotting was used to analyse the A beta secretion and MTT assay to observe the cell viability's change after being stably transfected with the plasmids by lipofectamine method.

Results: Stable transfectants were selected in 0.6 g.L-1 G418. delta APP695 cDNA construct transfection decreased significantly the production of beta amyloid in the SH-SY5Y compared with APP695 cDNA construct. It did not affect the cell viability by MTT method.

Conclusion: This result suggests that the alternative splicing of APP exons 2 and 3 modulates the secretion of A beta by influencing the processing of APP.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Amyloid beta-Peptides / metabolism*
Amyloid beta-Protein Precursor / genetics*
Blotting, Western
Exons
Gene Deletion*
Humans
Plasmids
Transfection
Tumor Cells, Cultured

Substances

Amyloid beta-Peptides
Amyloid beta-Protein Precursor