A simplified method for purification of annexin V from human placenta

Prep Biochem Biotechnol. 2003 Aug;33(3):209-15. doi: 10.1081/PB-120022989.

Abstract

A simplified procedure for purification of annexin V from human placenta was developed. At first, the protein was separated from other proteins in membrane bound form in the presence of Ca2+, then was extracted with EDTA and purified by affinity chromatography on PAAG-immobilized phosphatidylserine. The purified protein gave a single band with a molecular weight of 35,000 in SDS-PAGE.

MeSH terms

  • Annexin A5 / chemistry
  • Annexin A5 / isolation & purification*
  • Chromatography, Affinity
  • Female
  • Humans
  • Placenta / chemistry*
  • Pregnancy

Substances

  • Annexin A5