Determination of HBsAg subtypes in Western Siberian part of Russia

J Med Virol. 2003 Oct;71(2):183-7. doi: 10.1002/jmv.10468.

Abstract

A set of monoclonal antibodies with specificity for hepatitis B surface antigen (HBsAg) was used for subtyping this antigen in sera from indigenous natives, blood donors, and drug users in Western Siberia with a modified commercial enzyme immunoassay kit for HBsAg detection. Three subtypes of HBsAg in a ratio of 36 (78%) ayw2:8 ayw3varB (18%):2 (4%) adw2 were found in 46 (100%) HBsAg-positive sera of different aboriginal populations of Western Siberia: the Tundra Nenets, Northern Khanty, Southern Altaians, and Kazakhs. Four subtypes of HBsAg in a ratio of 81 (57%) ayw2:58 (15 ayw3varA and 43 ayw3varB; 44%):2 (1%) adw2 were detected in 141 (100%) samples of blood donors from ten cities of Western Siberia. Three subtypes of HBsAg in a ratio of 34 ayw3:(both variants, 33 ayw3varA and 1 ayw3varB; 97.1%):1 (2.9%) ayw2 were found in blood of 35 injection drug users in Novosibirsk.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Antibodies, Monoclonal / immunology
  • Antibody Specificity
  • Blood Donors
  • Hepatitis B Antibodies / immunology
  • Hepatitis B Surface Antigens / blood*
  • Hepatitis B Surface Antigens / classification*
  • Hepatitis B Surface Antigens / immunology
  • Hepatitis B virus / classification
  • Hepatitis B virus / immunology
  • Hepatitis B, Chronic / epidemiology*
  • Hepatitis B, Chronic / immunology
  • Hepatitis B, Chronic / virology
  • Humans
  • Immunoenzyme Techniques
  • Prevalence
  • Reagent Kits, Diagnostic
  • Siberia / epidemiology
  • Substance Abuse, Intravenous / complications

Substances

  • Antibodies, Monoclonal
  • Hepatitis B Antibodies
  • Hepatitis B Surface Antigens
  • Reagent Kits, Diagnostic