Light emitting diode irradiation protect against the amyloid beta 25-35 induced apoptosis of PC12 cell in vitro

Rui Duan; Ling Zhu; Timon Cheng-Yi Liu; Yan Li; Jiang Liu; Jianling Jiao; Xiaoyang Xu; Libo Yao; Songhao Liu

doi:10.1002/lsm.10216

Light emitting diode irradiation protect against the amyloid beta 25-35 induced apoptosis of PC12 cell in vitro

Lasers Surg Med. 2003;33(3):199-203. doi: 10.1002/lsm.10216.

Authors

Rui Duan¹, Ling Zhu, Timon Cheng-Yi Liu, Yan Li, Jiang Liu, Jianling Jiao, Xiaoyang Xu, Libo Yao, Songhao Liu

Affiliation

¹ Laboratory of Laser Sports Medicine, College of Sports Science, South China Normal University, Guangzhou, 510631, China.

PMID: 12949950
DOI: 10.1002/lsm.10216

Abstract

Background and objectives: Whether light emitting diode (LED) irradiation has effects on "beta-amyloid (A beta) induced apoptosis," a leading hypothesis of the cause of Alzheimer's disease (AD), or not?

Study design/materials and methods: Monolayer cell cultures of PC12 were subjected to A beta or/and LED irradiation at various intensity. Cell apoptosis was confirmed by morphological criteria, DNA fragmentation assay, and FAScan flow cytometer assay.

Results: Treatment of the cells with LED irradiation significantly diminished A beta induced apoptosis within 24 hours.

Conclusions: The LED irradiation, when utilized at power of 0.9 W/m(2) and 60 minutes has significantly diminished A beta induced apoptosis of PC12 cells.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Amyloid beta-Peptides / physiology*
Animals
Apoptosis / physiology*
Apoptosis / radiation effects*
Dose-Response Relationship, Radiation
Light*
PC12 Cells
Peptide Fragments / physiology*
Rats
Time Factors

Substances

Amyloid beta-Peptides
Peptide Fragments
amyloid beta-protein (25-35)